Document Type
Article
Publication Date
11-14-2017
Abstract
Tumor-sequencing studies have revealed the widespread genetic diversity of melanoma. Sequencing of 108 genes previously implicated in melanomagenesis was performed on 462 patient-derived xenografts (PDXs), cell lines, and tumors to identify mutational and copy number aberrations. Samples came from 371 unique individuals: 263 were naive to treatment, and 108 were previously treated with targeted therapy (34), immunotherapy (54), or both (20). Models of all previously reported major melanoma subtypes (BRAF, NRAS, NF1, KIT, and WT/WT/WT) were identified. Multiple minor melanoma subtypes were also recapitulated, including melanomas with multiple activating mutations in the MAPK-signaling pathway and chromatin-remodeling gene mutations. These well-characterized melanoma PDXs and cell lines can be used not only as reagents for a large array of biological studies but also as pre-clinical models to facilitate drug development.
Recommended Citation
Garman, Bradley; Anastopoulos, Ioannis N.; Krepler, Clemens; Brafford, Patricia; Sproesser, Katrin; Jiang, Yuchao; Wubbenhorst, Bradley; Amaravadi, Ravi; Bennett, Joseph; Beqiri, Marilda; Elder, David; Flaherty, Keith T.; Frederick, Dennie T.; Gangadhar, Tara C.; Guarino, Michael; Hoon, David; Karakousis, Giorgos; Liu, Qin; Mitra, Nandita; Petrelli, Nicholas J.; Schuchter, Lynn; Shannan, Batool; Shields, Carol L.; Wargo, Jennifer; Wenz, Brandon; Wilson, Melissa A.; Xiao, Min; Xu, Wei; Xu, Xaiowei; Yin, Xiangfan; Zhang, Nancy R.; Davies, Michael A.; Herlyn, Meenhard; and Nathanson, Katherine L., "Genetic and Genomic Characterization of 462 Melanoma Patient-Derived Xenografts, Tumor Biopsies, and Cell Lines." (2017). Wills Eye Hospital Papers. Paper 78.
https://jdc.jefferson.edu/willsfp/78
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
PubMed ID
29141224
Comments
This article has been peer reviewed. It is the author’s final published version in Cell Reports
Volume 21, Issue 7, November 2017, Pages 1936-1952
The published version is available at DOI: 10.1016/j.celrep.2017.10.052. Copyright © Garman et al.