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Description

Introduction

  • Pancreatic ductal adenocarcinoma (PDA) remains a deadly disease with a 5-year survival of 8% for all stages combined (Siegel RL et al.). Currently, it’s the third leading cause of cancer-related deaths in the United States and by 2020 it is projected to become the second leading cause (Rahib L, et al).
  • The poor prognosis in PDA is in part due to the limited therapies that are currently available.
  • This highlights the importance of high-throughput technologies for gene expression analyses and drug screens.
  • A recently developed NanoString Technologies nCounter® analysis system, an example of high-throughput technology, utilizes a color-coded barcode to directly measure multiple mRNA transcripts simultaneously.
  • Our ability to study RNA expression has classically been limited by low detection rate (microarrays) or high cost (RNA-Seq).
  • We utilized the nCounter® analysis system to measure changes in cancer-associated gene expression in PDA cell lines caused by different drug treatment.
  • We then validated these results using traditional assays to evaluate the sensitivity of the nCounter® analysis system.

Poster presented at: Metro Philadelphia Chapter of the American College of Surgeons in Philadelphia PA, United States.

Publication Date

5-22-2000

Keywords

Evaluating the Sensitivity of the NanoStrong nCounter® Analysis System to Determine Gene Expression Changes Associated with Chemotherapy Treatment, poster, Department of Surgery, Thomas Jefferson University

Disciplines

Surgery

Evaluating the Sensitivity of the NanoStrong nCounter® Analysis System to  Determine Gene Expression Changes Associated with Chemotherapy Treatment

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