Aptamer-hybrid nanoparticle bioconjugate efficiently delivers miRNA-29b to non-small-cell lung cancer cells and inhibits growth by downregulating essential oncoproteins.
MicroRNAs (miRNAs) are potentially attractive candidates for cancer therapy. However, their therapeutic application is limited by lack of availability of an efficient delivery system to stably deliver these potent molecules intracellularly to cancer cells while avoiding healthy cells. We developed a novel aptamer-hybrid nanoparticle bioconjugate delivery system to selectively deliver miRNA-29b to MUC1-expressing cancer cells. Significant downregulation of oncoproteins DNMT3b and MCL1 was demonstrated by these MUC1 aptamer-functionalized hybrid nanoparticles in A549 cells. Furthermore, downregulation of these oncoproteins led to antiproliferative effect and induction of apoptosis in a superior version when compared with Lipofectamine 2000. This novel aptamer-hybrid nanoparticle bioconjugate delivery system could potentially serve as a platform for intracellular delivery of miRNAs to cancer cells, hence improving the therapeutic outcome of lung cancer.
Perepelyuk, Maryna; Maher, Christina; Lakshmikuttyamma, Ashakumary; and Shoyele, Sunday A., "Aptamer-hybrid nanoparticle bioconjugate efficiently delivers miRNA-29b to non-small-cell lung cancer cells and inhibits growth by downregulating essential oncoproteins." (2016). College of Pharmacy Faculty Papers. Paper 27.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License
This article has been peer reviewed. It is the author’s final published version in International Journal of Nanomedicine by Dove Medical Press
Volume 11, July 2016, Pages 3533-3544
The published version is available at DOI: 10.2147/IJN.S110488. Copyright © Perepelyuk et al.