Thioaptamer conjugated liposomes for tumor vasculature targeting.

Authors

Aman P Mann, University of Texas Health Science Center at HoustonFollow
Rohan C Bhavane, University of Texas Health Science Center at Houston
Anoma Somasunderam, University of Texas Health Science Center at HoustonFollow
Brenda Liz Montalvo-Ortiz, Thomas Jefferson University
Ketan B Ghaghada, University of Texas Health Science Center at Houston
David Volk, University of Texas Health Science Center at Houston
René Nieves-Alicea, The John Theurer Cancer Center, Hackensack University Medical Center, Hackensack, New JerseyFollow
K Stephen Suh, The John Theurer Cancer Center, Hackensack University Medical Center, Hackensack, New JerseyFollow
Mauro Ferrari, The Methodist Hospital Research Institute, Houston, TexasFollow
Ananth Annapragada, University of Texas Health Science Center at Houston
David G Gorenstein, University of Texas Health Science Center at HoustonFollow
Takemi Tanaka, Department of Pharmaceutical Sciences, Thomas Jefferson University, Philadelphia, PAFollow

Document Type

Article

Publication Date

4-1-2011

Comments

This article has been peer reviewed. It was published in: Oncotarget

Volume 2, Issue 4, April 2011, Pages 298-304.

The published version is available at PMID: 21666286. Copyright © Impact Journals LLC

Abstract

Recent developments in multi-functional nanoparticles offer a great potential for targeted delivery of therapeutic compounds and imaging contrast agents to specific cell types, in turn, enhancing therapeutic effect and minimizing side effects. Despite the promise, site specific delivery carriers have not been translated into clinical reality. In this study, we have developed long circulating liposomes with the outer surface decorated with thioated oligonucleotide aptamer (thioaptamer) against E-selectin (ESTA) and evaluated the targeting efficacy and PK parameters. In vitro targeting studies using Human Umbilical Cord Vein Endothelial Cell (HUVEC) demonstrated efficient and rapid uptake of the ESTA conjugated liposomes (ESTA-lip). In vivo, the intravenous administration of ESTA-lip resulted in their accumulation at the tumor vasculature of breast tumor xenografts without shortening the circulation half-life. The study presented here represents an exemplary use of thioaptamer and liposome and opens the door to testing various combinations of thioaptamer and nanocarriers that can be constructed to target multiple cancer types and tumor components for delivery of both therapeutics and imaging agent.

Recommended Citation

Mann, Aman P; Bhavane, Rohan C; Somasunderam, Anoma; Liz Montalvo-Ortiz, Brenda; Ghaghada, Ketan B; Volk, David; Nieves-Alicea, René; Suh, K Stephen; Ferrari, Mauro; Annapragada, Ananth; Gorenstein, David G; and Tanaka, Takemi, "Thioaptamer conjugated liposomes for tumor vasculature targeting." (2011). College of Pharmacy Faculty Papers. Paper 19.
https://jdc.jefferson.edu/pharmacyfp/19

PubMed ID

21666286