Assessing the utilization of high-resolution 2-field HLA typing in solid organ transplantation.

Authors

Yanping Huang, Thomas Jefferson University; The Children’s Hospital of PhiladelphiaFollow
Anh Dinh, The Children’s Hospital of Philadelphia
Steven Heron, The Children’s Hospital of Philadelphia
Allison Gasiewski, The Children’s Hospital of Philadelphia
Carolina Kneib, The Children’s Hospital of Philadelphia
Hilary Mehler, The Children’s Hospital of Philadelphia
Michael T. Mignogno, The Children’s Hospital of Philadelphia
Ryan Morlen, The Children’s Hospital of Philadelphia
Larissa Slavich, The Children’s Hospital of Philadelphia
Ethan Kentzel, The Children’s Hospital of Philadelphia
Edward C. Frackelton, The Children’s Hospital of Philadelphia
Jamie L. Duke, The Children’s Hospital of Philadelphia
Deborah Ferriola, The Children’s Hospital of Philadelphia
Timothy Mosbruger, The Children’s Hospital of Philadelphia
Olga A. Timofeeva, Temple University
Steven S. Geier, Temple University; University of Pennsylvania
Dimitri Monos, The Children’s Hospital of Philadelphia;

Document Type

Article

Publication Date

1-8-2019

Comments

This is the peer reviewed version of the following article: Huang, Y., Dinh, A., Heron, S., Gasiewski, A., Kneib, C., Mehler, H., Mignogno, M.T., Morlen, R., Slavich, L., Kentzel, E., Frackelton, E.C., Duke, J.L., Ferriola, D., Mosbruger, T., Timofeeva, O.A., Geier, S.S., & Monos, D. (2019). Assessing the utilization of high-resolution 2-field HLA typing in solid organ transplantation. American Journal of Transplantation, which has been published in final form at DOI: 10.1111/ajt.15258. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Abstract

HLA typing in solid organ transplantation (SOT) is necessary for determining HLA-matching status between donor-recipient pairs and assessing patients' anti-HLA antibody profiles. Histocompatibility has traditionally been evaluated based on serologically defined HLA antigens. The evolution of HLA typing and antibody identification technologies, however, has revealed many limitations with using serologic equivalents for assessing compatibility in SOT. The significant improvements to HLA typing introduced by next-generation sequencing (NGS) require an assessment of the impact of this technology on SOT. We have assessed the role of high-resolution 2-field HLA typing (HR-2F) in SOT by retrospectively evaluating NGS-typed pre- and post-SOT cases. HR-2F typing was highly instructive or necessary in 41% (156/385) of the cases. Several pre- and posttransplant scenarios were identified as being better served by HR-2F typing. Five different categories are presented with specific case examples. The experience of another center (Temple University Hospital) is also included, whereby 21% of the cases required HR-2F typing by Sanger sequencing, as supported by other legacy methods, to properly address posttransplant anti-HLA antibody issues.

Recommended Citation

Huang, Yanping; Dinh, Anh; Heron, Steven; Gasiewski, Allison; Kneib, Carolina; Mehler, Hilary; Mignogno, Michael T.; Morlen, Ryan; Slavich, Larissa; Kentzel, Ethan; Frackelton, Edward C.; Duke, Jamie L.; Ferriola, Deborah; Mosbruger, Timothy; Timofeeva, Olga A.; Geier, Steven S.; and Monos, Dimitri, "Assessing the utilization of high-resolution 2-field HLA typing in solid organ transplantation." (2019). Department of Pathology, Anatomy, and Cell Biology Faculty Papers. Paper 269.
https://jdc.jefferson.edu/pacbfp/269

PubMed ID

30623581

Language

English