Document Type

Article

Publication Date

3-15-2024

Comments

This article is the author’s final published version in iScience, Volume 27, Issue 3, March 2024, Article number 109119.

The published version is available at https://doi.org/10.1016/j.isci.2024.109119. Copyright © 2024 The Author(s).

Publication made possible in part by support from the Jefferson Open Access Fund

Abstract

In a previous report, keratinocytes were shown to share their gene expression profile with surrounding Langerhans cells (LCs), influencing LC biology. Here, we investigated whether transferred material could substitute for lost gene products in cells subjected to Cre/Lox conditional gene deletion. We found that in human Langerin-Cre mice, epidermal LCs and CD11b+CD103+ mesenteric DCs overcome gene deletion if the deleted gene was expressed by neighboring cells. The mechanism of material transfer differed from traditional antigen uptake routes, relying on calcium and PI3K, being susceptible to polyguanylic acid inhibition, and remaining unaffected by inflammation. Termed intracellular monitoring, this process was specific to DCs, occurring in all murine DC subsets tested and human monocyte-derived DCs. The transferred material was presented on MHC-I and MHC-II, suggesting a role in regulating immune responses.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

Additional Files
Document S1. Figures S1‒S6.pdf (4022 kB)

PubMed ID

38384841

Language

English

Download

Share

COinS