Mitochondrial lysyl-tRNA synthetase independent import of tRNA lysine into yeast mitochondria.

Authors

Naresh Babu V Sepuri, University of Hyderabad; Thomas Jefferson UniversityFollow
Madhavi Gorla, University of HyderabadFollow
Michael P King, Thomas Jefferson UniversityFollow

Document Type

Article

Publication Date

1-1-2012

Comments

This article is peer reviewed. The original version is published in PLoS ONE, 2012;7(4):e35321. ©PLoS.org

Abstract

Aminoacyl tRNA synthetases play a central role in protein synthesis by charging tRNAs with amino acids. Yeast mitochondrial lysyl tRNA synthetase (Msk1), in addition to the aminoacylation of mitochondrial tRNA, also functions as a chaperone to facilitate the import of cytosolic lysyl tRNA. In this report, we show that human mitochondrial Kars (lysyl tRNA synthetase) can complement the growth defect associated with the loss of yeast Msk1 and can additionally facilitate the in vitro import of tRNA into mitochondria. Surprisingly, the import of lysyl tRNA can occur independent of Msk1 in vivo. This suggests that an alternative mechanism is present for the import of lysyl tRNA in yeast.

Recommended Citation

Sepuri, Naresh Babu V; Gorla, Madhavi; and King, Michael P, "Mitochondrial lysyl-tRNA synthetase independent import of tRNA lysine into yeast mitochondria." (2012). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 40.
https://jdc.jefferson.edu/bmpfp/40

PubMed ID

22539966