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This article is the author’s final published version in STAR Protocols, Volume 4, Issue 1, January 2023, Article number 101993.

The published version is available at Copyright © Karliner and Merry.


Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate PC12 cells into equivalent neurite densities through live-cell imaging for morphological, immunocytochemical, and biochemical analyses. We detail steps on optimized substrate coating, plating techniques, culture media, validation steps, and quantification techniques.

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Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

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