Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate PC12 cells into equivalent neurite densities through live-cell imaging for morphological, immunocytochemical, and biochemical analyses. We detail steps on optimized substrate coating, plating techniques, culture media, validation steps, and quantification techniques.
Karliner, Jordyn and Merry, Diane E, "Differentiating PC12 Cells to Evaluate Neurite Densities Through Live-Cell Imaging" (2023). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 232.
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