Purification and Use of tRNA for Enzymatic Post-translational Addition of Amino Acids to Proteins.

Authors

Irem Avcilar-Kucukgoze, University of Pennsylvania; Thomas Jefferson University
Howard Gamper, Thomas Jefferson UniversityFollow
Ya-Ming Hou, Thomas Jefferson UniversityFollow
Anna Kashina, University of Pennsylvania

Document Type

Article

Publication Date

12-9-2020

Comments

This article is the authors’ final published version in STAR Protocols, Volume 1, Issue 3, December 2020, Article number 100207.

The published version is available at https://doi.org/10.1016/j.xpro.2020.100207. Copyright © Avcilar-Kucukgoze et al.

Abstract

Post-translational addition of amino acids to proteins by enzymes using aminoacyl-tRNA is an emerging regulatory mechanism. Examples include Arg transfer in eukaryotes, Leu/Phe transfer in bacteria, and tRNA-synthetase-mediated addition of amino acids to Lys side chains. Here, we present a method of purification and use of tRNA for such reactions, focusing on tRNA^Arg and its use for arginylation. This method can also be used for other tRNA-mediated reactions. For complete details on the use and execution of this protocol, please refer to Avcilar-Kucukgoze et al. (2020).

Recommended Citation

Avcilar-Kucukgoze, Irem; Gamper, Howard; Hou, Ya-Ming; and Kashina, Anna, "Purification and Use of tRNA for Enzymatic Post-translational Addition of Amino Acids to Proteins." (2020). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 187.
https://jdc.jefferson.edu/bmpfp/187

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

PubMed ID

33377101

Language

English