Event Title

Session #4: Expression and Function of Cyclic Phosphate-containing RNAs: a Hidden Layer of the Transcriptome

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Event Website

https://cm.jefferson.edu/big-data-september-2020/

Start Date

9-9-2020 9:10 AM

End Date

9-9-2020 9:40 AM

Description

Cellular RNA molecules contain phosphate or hydroxyl ends. A 2′,3′-cyclic phosphate (cP) is one of the 3′-terminal forms of RNAs mainly generated from RNA cleavage by ribonucleases. Although transcriptome profiling using RNA-seq has become a ubiquitous tool in biomedical research, cP-containing RNAs (cP-RNAs) form a hidden layer of transcriptome, because standard RNA-seq is unable to capture them. Despite cP-RNAs’ invisibility in RNA-seq data, increasing evidence indicates that they are not accumulated simply as non-functional degradation products; rather, they have physiological roles in various biological processes, designating them as noteworthy functional molecules. We will present a comprehensive, genome-wide identification of short cP-RNA expression repertoire in cell lines and tissues, which has been achieved by our developed cP-RNA-seq. Our experimental data on the expressional regulation and functional roles of cP-RNAs in cancers, asthma, and infectious diseases will be further presented and discussed.

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Presentation: 26:41

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Sep 9th, 9:10 AM Sep 9th, 9:40 AM

Session #4: Expression and Function of Cyclic Phosphate-containing RNAs: a Hidden Layer of the Transcriptome

Cellular RNA molecules contain phosphate or hydroxyl ends. A 2′,3′-cyclic phosphate (cP) is one of the 3′-terminal forms of RNAs mainly generated from RNA cleavage by ribonucleases. Although transcriptome profiling using RNA-seq has become a ubiquitous tool in biomedical research, cP-containing RNAs (cP-RNAs) form a hidden layer of transcriptome, because standard RNA-seq is unable to capture them. Despite cP-RNAs’ invisibility in RNA-seq data, increasing evidence indicates that they are not accumulated simply as non-functional degradation products; rather, they have physiological roles in various biological processes, designating them as noteworthy functional molecules. We will present a comprehensive, genome-wide identification of short cP-RNA expression repertoire in cell lines and tissues, which has been achieved by our developed cP-RNA-seq. Our experimental data on the expressional regulation and functional roles of cP-RNAs in cancers, asthma, and infectious diseases will be further presented and discussed.

https://jdc.jefferson.edu/biginbigdata/2020/sep9/4