WE COMPARED LUNG DELIVERY METHODS OF RECOMBINANT ADENOVIRUS (RAD): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2-4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.
Recommended CitationKazzaz, Jeffrey A; Strayer, Marlene S; Wu, Jichuan; Malone, Daniel J; Koo, Hshi-Chi; Shaffer, Thomas H; Davis, Jonathan M; Strayer, David S; and Wolfson, Marla R, "Perfluorochemical liquid-adenovirus suspensions enhance gene delivery to the distal lung." (2011). Department of Pathology, Anatomy and Cell Biology Faculty Papers. Paper 138.