Document Type

Article

Publication Date

8-18-2011

Comments

This article has been peer reviewed. It was published in: Pulmonary Medicine.

2011, Article number 918036.

The published version is available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159382/. DOI: 10.1155/2011/918036.

Copyright © 2011 Jeffrey A. Kazzaz et al.

Abstract

WE COMPARED LUNG DELIVERY METHODS OF RECOMBINANT ADENOVIRUS (RAD): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2-4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.

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