Document Type
Article
Publication Date
1-24-2017
Abstract
T cells are the most abundant cell type found in afferent lymph, but their migration through lymphatic vessels (LVs) remains poorly understood. Performing intravital microscopy in the murine skin, we imaged T cell migration through afferent LVs in vivo. T cells entered into and actively migrated within lymphatic capillaries but were passively transported in contractile collecting vessels. Intralymphatic T cell number and motility were increased during contact-hypersensitivity-induced inflammation and dependent on ICAM-1/LFA-1 interactions. In vitro, blockade of endothelial cell-expressed ICAM-1 reduced T cell adhesion, crawling, and transmigration across lymphatic endothelium and decreased T cell advancement from capillaries into lymphatic collectors in skin explants. In vivo, T cell migration to draining lymph nodes was significantly reduced upon ICAM-1 or LFA-1 blockade. Our findings indicate that T cell migration through LVs occurs in distinct steps and reveal a key role for ICAM-1/LFA-1 interactions in this process.
Recommended Citation
Teijeira, Alvaro; Hunter, Morgan C.; Russo, Erica; Proulx, Steven T; Frei, Thomas; Debes, Gudrun F.; Coles, Marc; Melero, Ignacio; Detmar, Michael; Rouzaut, Ana; and Halin, Cornelia, "T Cell Migration from Inflamed Skin to Draining Lymph Nodes Requires Intralymphatic Crawling Supported by ICAM-1/LFA-1 Interactions." (2017). Department of Microbiology and Immunology Faculty Papers. Paper 87.
https://jdc.jefferson.edu/mifp/87
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
PubMed ID
28122237
Comments
This article has been peer reviewed. It is the author’s final published version in Cell Reports
Volume 18, Issue 4, January 2017, Pages 857-865
The published version is available at DOI: 10.1016/j.celrep.2016.12.078. Copyright © Teijeira et al.