Use of an Anaerobic Chamber Environment for the Assay of Endogenous Cellular Protein-Tyrosine Phosphatase Activities.

Authors

Li Zhu, Thomas Jefferson University
Barry Goldstein, Thomas Jefferson UniversityFollow

Document Type

Article

Publication Date

6-11-2002

Comments

This article has been peer reviewed. It was published in: Biological procedures online

2002 Jun 11;4:1-9.

The published version is available at DOI: 10.1251/bpo28. Copyright © BioMed Central

Abstract

Protein-tyrosine phosphatases (PTPases) have a catalytic cysteine residue whose reduced state is integral to the reaction mechanism. Since exposure to air can artifactually oxidize this highly reactive thiol, PTPase assays have typically used potent reducing agents to reactivate the enzymes present; however, this approach does not allow for the measurement of the endogenous PTPase activity directly isolated from the in vivo cellular environment. Here we provide a method for using an anaerobic chamber to preserve the activity of the total PTPase complement in a tissue lysate or of an immunoprecipitated PTPase homolog to characterize their endogenous activation state. Comparison with a sample treated with biochemical reducing agents allows the determination of the activatable (reducible) fraction of the endogenous PTPase pool.

Recommended Citation

Zhu, Li and Goldstein, Barry, "Use of an Anaerobic Chamber Environment for the Assay of Endogenous Cellular Protein-Tyrosine Phosphatase Activities." (2002). Department of Medicine Faculty Papers. Paper 92.
https://jdc.jefferson.edu/medfp/92

PubMed ID

12734574