Document Type
Article
Publication Date
July 2007
Abstract
Immunotherapy holds great promise for treatment of infectious and malignant diseases and might help to prevent the occurrence and recurrence of cancer. We produced a plant-derived tumor-associated colorectal cancer antigen EpCAM (pGA733) at high yields using two modern plant expression systems. The full antigenic domain of EpCAM was efficiently purified to confirm its antigenic and immunogenic properties as compared to those of the antigen expressed in the baculovirus system (bGA733). Recombinant plant-derived antigen induced a humoral immune response in BALB/c mice. Sera from those mice efficiently inhibited the growth of SW948 colorectal carcinoma cells xenografted in nude mice, as compared to the EpCAM-specific mAb CO17-1A. Our results support the feasibility of producing anti-cancer recombinant vaccines using plant expression systems.
Recommended Citation
Brodzik, Robert; Spitsin, Sergei; Golovkin, Max; Bandurska, Katarzyna; Portocarrero, Carla; Okulicz, Monika; Steplewski, Zenon; and Koprowski, Hilary, "Plant-derived EpCAM antigen induces protective anti-cancer response." (2007). Department of Cancer Biology Faculty Papers. Paper 15.
https://jdc.jefferson.edu/cbfp/15
Comments
This article has been peer reviewed. It is the authors’ final version prior to publication in Cancer Immunology, Immunotherapy: CII 57(3):317-323, March 2008, e-published ahead of print July 19, 2007. Copyright © 2007 Springer-Verlag. The published version is available at DOI: 10.1007/s00262-007-0366-4.